Sepiatec’s new Prep SFC 360 system is a compact yet large-scale unit designed for preparative separations using supercritical fluid chromatography. The unit has been designed for flow rates of up to 360 ml of supercritical fluid per minute. Depending on the modifier portion, even up to 600 ml/min may be achieved (in the case of a modifier portion of 40 %).
All of the advantages of the SFC technology are combined: Quick separation, a reduced consumption of organic solvents and thus cost, increased safety and an environmentally friendly process. In spite of its remarkable capacity, the Prep SFC 360 system features a compact design with measurements of 150 x 68 x 178 cm (W x D x H), so that integration within a walk-in laboratory hood does not create any problem. Additionally, below the unit, several large fraction vessels or a safety cabinet for solvents may be placed.
A CO2 - as well as a modifier pump are provided, each of them equipped with a 360 ml pump head and approved for a pressure of 360 bar. Up to three different organic organic modifiers can be mixed and used for separations.
Beside the column mounting, the injection valve and the UV flow cell are also integrated within the column oven, which is heatable up to 50 °C. Separation columns with inner diameters between 25 and 50 mm and a length of up to 800 mm can be used.
When working in “Single Mode“, up to 8 fractions can be collected (CO2 flow < 180 ml/min); in “Tandem Mode“ 4 fractions are available (CO2 flow > 180 ml/min).
The collection of the fractions is performed in unpressurized state; therefore screening of the vessels for reasons of safety is not necessary. The fraction volume in the Prep SFC 360 system is not limited. Fractions of any size may be collected and simply be tapped for further processing.
|Operating mode||1 preparative column CO2 and organic solvents|
|Solvents||CO2 and organic solvents|
|Number of modifier solvents||3 solvents, mixable|
|Total flow rates||Up to 600 ml/min (40 % modifier)|
|CO2 pump||360 ml pump head, 360 bar|
|Modifier pump||360 ml pump head, 360 bar|
|Operating pressure||Up to 300 bar, adjustable online backpressure control|
|Injection||Total loop with syringe pump|
|Columns||25 to 50 mm ID up to 800 mm length|
|Column oven||Up to 50 °C, integrated heating module, injection valve and UV flow cell|
|Detection||UV detector, wavelength 190 to 500 nm|
|Fraction collection||1 to 8 fractions (single mode), 1 to 4 fractions (tandem mode)|
|Software||Prep SFC 360 control software|
|System controller||Integrated PC, 15“ TFT touch-screen|
|Connections||3 USB ports, ethernet|
|Dimensions||150 x 68 x 178 cm (W x D x H)|
|INTEGRATED WATER COOLED CHILLER||Huber Unichiller 015-MPC plus (or successor model)|
|Temperature range||- 20 °C to 40 °C|
|Temperature consistency||± 0.5 °C|
|Cooling performance||15 °C / 1.5 KW, 0 °C / 1.0 KW, - 10 °C / 0.7 KW, - 20 °C / 0.3 KW|
|Cooling device||°C / 0.7 KW, - 20 °C / 0.3 KW Air-cooled, CFC- and HCFC-free|
|Dimensions||42 x 48 x 58 cm (W x D x H)|